PATTERNS OF INFECTIONS AND ANTIMICROBIAL RESISTANCE AMONG GRAM NEGATIVE ORGANISMS IN A TERTIARY CARE HOSPITAL

Priya  Kumari; Anamika  Singh; Priyanka Paul  Biswas; Aninda  Sen

doi:10.51168/sjhrafrica.v6i3.1584

Authors

Priya Kumari M. Sc. student, Department of Microbiology, Katihar Medical College, Al-Karim University, Katihar, Bihar, India, PIN: 854106. Mob: +91-62049 45660;
Anamika Singh Associate Professor, Department of Microbiology, Katihar Medical College, Al-Karim University, Katihar, Bihar, India, PIN: 854106. Mob: +91-79911 49177;
Priyanka Paul Biswas Professor, Department of Microbiology, Katihar Medical College, Al-Karim University, Katihar, Bihar, India, PIN: 854106. Mob: +91-83403 94124;
Aninda Sen Professor, Department of Microbiology, Katihar Medical College, Al-Karim University, Katihar, Bihar, India, PIN: 854106. Mob: +91-94312 06029;

DOI:

https://doi.org/10.51168/sjhrafrica.v6i3.1584

Keywords:

multidrug resistance (MDR), indoor patient department (IPD), outdoor patient department (OPD)

Abstract

Introduction

Regional variation in the distribution of various organisms is noted. Monitoring the use of antimicrobials and constant review of sensitivity patterns is imperative. The main aim of this study was to determine the species prevalence of Gram-negative isolates, including antibiotic resistance patterns, from various clinical specimens.

Materials and methods

A hospital-based observational descriptive study was conducted to identify and perform an antibiotic susceptibility test of Gram-negative bacilli from clinical samples. The VITEK 2 Compact (30 card capacity) system was used, where a fluorogenic methodology for organism identification and a turbidimetric method for susceptibility testing was performed. The method used for antimicrobial susceptibility testing was the doubling dilution technique for MIC based on the microdilution method.

Results

A total of 970 clinical samples were received, of which culture positivity was seen in 391(40.3%) cases. The most common specimen was urine at 35.1% (45/128), followed by a blood sample at 27.3% (35/128). The most common isolate was Escherichia coli 36.0% (47/128), followed by Klebsiella pneumoniae 16.4% (21/128) and Burkholderia cepacia 11.7% (15/128). Among the IPD patients, Escherichia coli showed maximum sensitivity to amikacin 67.7% and gentamicin 61.2%; 55.5% of the strains of Acinetobacter baumannii were sensitive to ceftazidime. Pseudomonas aeruginosa was sensitive to cefepime, cefoperazone/sulbactam, and levofloxacin, 70.0% each respectively. 86.6% of strains of Burkholderia cepacia retained susceptibility to meropenem.

Conclusion

This study isolates (lactose-fermenting bacteria) were sensitive to trimethoprim-sulfamethoxazole, levofloxacin, and amoxicillin/clavulanic acid. On the other hand, the non-lactose-fermenting bacteria were susceptible to imipenem, meropenem, and levofloxacin.

Recommendation

Recommendations include strongly preferred alternative drugs for active or combination treatments.

Author Biographies

Priya Kumari, M. Sc. student, Department of Microbiology, Katihar Medical College, Al-Karim University, Katihar, Bihar, India, PIN: 854106. Mob: +91-62049 45660;

ORCID: https://orcid.org/0009-0005-2334-1531

Anamika Singh, Associate Professor, Department of Microbiology, Katihar Medical College, Al-Karim University, Katihar, Bihar, India, PIN: 854106. Mob: +91-79911 49177;

ORCID: https://orcid.org/ 0000-0001-7394-5687

Priyanka Paul Biswas, Professor, Department of Microbiology, Katihar Medical College, Al-Karim University, Katihar, Bihar, India, PIN: 854106. Mob: +91-83403 94124;

ORCID: https://orcid.org/0000-0002-7226-8175

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