PREVALENCE, IDENTIFICATION AND ANTIMICROBIAL SUSCEPTIBILITY TESTING OF Acinetobacter baumannii complex & PESUDOMONAS SPECIES IN A TERTIARY CARE CENTRE IN EASTERN PART OF BIHAR.

Md Sahil  Anwar; Priyanka Paul  Biswas; Shyamasree  Nandy; Kahkashan  Akhter; Md. Nahidul  Wari; Aninda  Sen

doi:10.51168/sjhrafrica.v6i3.1585

Authors

Md Sahil Anwar PhD. Scholar, Department of Microbiology, Katihar Medical College, Al-Karim University, Katihar, Bihar, India, PIN: 854106. Mob: +91-79797 59986;
Priyanka Paul Biswas Professor, Department of Microbiology, Katihar Medical College, Al-Karim University, Katihar, Bihar, India, PIN: 854106. Mob: +91-83403 94124;
Shyamasree Nandy Demonstrator, Hamdard Institute of Medical Sciences, New Delhi, India, PIN: 110062. Mob: +91- 92130 84864;
Kahkashan Akhter Associate Professor, Department of Microbiology, Katihar Medical College, Al-Karim University, Katihar, Bihar, India, PIN: 854106. Mob: +91-99340 58224;
Md. Nahidul Wari Assistant Professor, Department of Microbiology, Katihar Medical College, Al-Karim University, Katihar, Bihar, India, PIN: 854106. Mob: +91-90138 44259;
Aninda Sen Professor, Department of Microbiology, Katihar Medical College, Al-Karim University, Katihar, Bihar, India, PIN: 854106. Mob: +91-94312 06029;

DOI:

https://doi.org/10.51168/sjhrafrica.v6i3.1585

Abstract

Introduction:

Acinetobacter baumannii and Pseudomonas species have emerged as a notorious pathogen causing health care associated infections with acquired antibiotic resistance that leaves few options for appropriate selection of drugs thereby complicating treatment.

Aim:

The goal of the study was to isolate, identify, and determine the antimicrobial susceptibility pattern of Acinetobacter baumannii and Pseudomonas species from various clinical samples in a tertiary care centre.

Materials & Methods:

A hospital-based cross-sectional study was conducted to identify and perform antibiotic susceptibility tests of non-lactose fermenting Gram-negative bacilli from clinical samples from November 2023 to September 2024 in our Microbiology laboratory at a tertiary centre in Northern Bihar. 60 consecutive isolates of Acinetobacter baumannii and Pseudomonas species were identified by the VITEK 2 gram-negative identification card. Antibiotic susceptibility testing was performed using the MIC microbroth doubling dilution technique, also by the same instrument.

Result:

A total of 1058 clinical samples from both outdoor and indoor patients were processed, out of which 186 yielded positive cultures. Amongst the 130 of these 186 (130/186) samples, Gram-negative bacteria Acinetobacter baumannii complex were isolated in 36 (36/130) samples, and Pseudomonas species in 23 of these 130 samples (23/130). The majority (30.8%) of the isolates were from the Medicine department 30.8%. Acinetobacter baumannii complex was mainly isolated from sputum samples, 44.4% (16/36), followed by 30.5% (11/36) from blood. 78% of strains of Pseudomonas aeruginosa isolated from sputum samples were sensitive to piperacillin/tazobactam, cefepime, meropenem, amikacin, and ciprofloxacin, respectively. 100% of the strains isolated from the pus sample showed resistance to ceftazidime, cefepime, and 90% to imipenem.

Conclusion:

In our study settings, nitrofurantoin is considered to be the best drug for the treatment of patients suffering from urinary tract infections, whereas in the case of Pseudomonas aeruginosa, piperacillin/tazobactam, followed by meropenem and nitrofurantoin, can be used for empirical treatment.

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