THE ROLE OF BACTERIAL TRANSLOCATION AND MICROBIOTA IN INTESTINAL BLOCKAGE: AN EXPERIMENTAL STUDY.

Jitendra Kumar  Ranjan; Pritam  Kar

doi:10.51168/sjhrafrica.v5i6.1204

Authors

Jitendra Kumar Ranjan MS, Assistant Professor, General Surgery, SNMMCH, Dhanbad, India
Pritam Kar Senior Resident, General Surgery, SNMMCH, Dhanbad, India

DOI:

https://doi.org/10.51168/sjhrafrica.v5i6.1204

Keywords:

Obstruction of the Intestines, Ischemia, Translocation of Bacteria, Rats

Abstract

Background:

The objective of this study is to investigate bacterial typing (BT) in the context of infectious diseases (I.O.), including the identification of pathogens, their transmission routes, and common characteristics.

Methods:

The study included 90 albino male rats in three groups: control (GI), simple ileal blockage (GII), and strangulated (GIII). Various surgical procedures were conducted under intramuscular ketamine anesthesia. Biochemical analysis measured CRP, IL-10, and oxidant and antioxidant activities. Histopathology assessed mucosal injury and inflammatory cell infiltration. Bacteriological examination identified bacterial species and CFU by cultivating tissue and blood samples.

Observation:

Strangulated intestinal obstruction increased bacterial proliferation, oxidative stress indicators, and IL-10 response, according to the study. The mean CFU/g of luminal bacteria count was 8.8 × 108 in GI, 5.6 × 1010 in GII, and 1.4 × 1012 in GIII. Mean MDA levels were 5.5 ± 0.5 in GI, 12.0 ± 1.9 in GII, and 21.9 ± 1.0 in GIII. The mean GPx levels were 125 ± 30 in GI, 165 ± 40 in GII, and 150 ± 35 in GIII. The mean IL-10 levels were 50 ± 15 in GI, 36 ± 12 in GII, and 9 ± 3 in GIII. Bacterial translocation was polymicrobial, with 49% in GII and 60% in GIII. Most enteric bacteria were E. coli, 42.2% in GII and 45.5% in GIII.

Conclusion:

Intestinal obstruction causes bidirectional bacterial translocation through a systemic portal, with a three-hit model primarily responsible for the ensuing inflammatory response. A non-selective marker of possible I.O. cases is the C-reactive protein. The C-reactive protein serves as an indicator of both Impaired blood flow to the tissues and the level of BT.

Recommendations:

Further studies should focus on developing more specific markers for early diagnosis of intestinal obstruction subtypes and exploring potential therapeutic interventions to manage bacterial translocation effectively