Clinico-Microbiological Profile and Antifungal Susceptibility of Subcutaneous Mycoses with Bone Involvement: A Cross-Sectional Study from a Tertiary Care Center in Eastern Odisha, India.

Sajid  Khan; Sasmita  Khatua; Madhumita  Swain

doi:10.51168/sjhrafrica.v7i3.2508

Authors

Sajid Khan Senior resident, Department of Microbiology, Fakir Mohan Medical College, Balasore, Odisha, India
Sasmita Khatua Associate Professor, Department of Microbiology, Pandit Raghunath Murmu Medical College, Baripada, Odisha, India
Madhumita Swain Assistant Professor, Department of Microbiology, DDMCH, Keonjhar, Odisha, India

DOI:

https://doi.org/10.51168/sjhrafrica.v7i3.2508

Keywords:

Antifungal susceptibility test, antifungal susceptibility, histopathology, fungal culture

Abstract

Background:

Subcutaneous mycoses are chronic fungal infections affecting the skin and subcutaneous tissues, commonly occurring in tropical regions among individuals engaged in outdoor occupations. Accurate identification and antifungal susceptibility testing are important for appropriate management due to emerging drug resistance.

Objective:

To determine the prevalence of subcutaneous mycoses, identify the causative fungal species, and evaluate antifungal susceptibility patterns among clinically suspected cases.

Methods:

This cross-sectional study was conducted from September 2021 to August 2023 among patients clinically suspected of subcutaneous mycoses attending a tertiary care center in Eastern Odisha, India. Clinical samples, including skin scrapings, tissue, pus, discharge, granules, and biopsy specimens, were collected aseptically. Samples were examined using a potassium hydroxide (KOH) mount, cultured on Sabouraud dextrose agar, and identified using standard microbiological techniques. Histopathological examination was performed using hematoxylin and eosin staining. Antifungal susceptibility testing was conducted using the microbroth dilution method.

Results:

Among 110 clinically suspected cases, subcutaneous mycoses were detected in 52.72% by culture, 78.18% by KOH mount, and 80% by histopathology. The most common isolates were Madurella spp. (48.27%), followed by Exophiala spp. (29.31%) and Fonsecaea spp. (22.41%). All isolates showed resistance to fluconazole and anidulafungin, while most isolates were sensitive to itraconazole, posaconazole, and amphotericin B.

Conclusion:

Subcutaneous mycoses remain prevalent in rural populations with occupational exposure. Antifungal susceptibility testing is essential for guiding therapy, with itraconazole

Author Biography

Sajid Khan, Senior resident, Department of Microbiology, Fakir Mohan Medical College, Balasore, Odisha, India

is a Senior Resident in the Department of Microbiology at Fakir Mohan Medical College, Odisha, with research interests in medical mycology and infectious diseases.

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