PREVALENCE AND SENSITIVITY PATTERN OF ESBL PRODUCERS IN DIFFERENT CLINICAL ISOLATES FROM A TERTIARY HEALTHCARE CENTER OF EASTERN INDIA, A CROSS-SECTIONAL STUDY.

Lipika Jena; Birsen  Behera; Diptimayee  Jena; Santosh  Singh; Rajashree  Panigrahy; Kundan Kumar  Sahu; Dattatreya  Kar; Suren Kumar  Das

doi:10.51168/sjhrafrica.v5i6.716

Authors

Lipika Jena IMS & SUM Hospital, Siksha O Anusandhan (Deemed to be University)
Birsen Behera IMS & SUM Hospital, Siksha O Anusandhan (Deemed to be University)
Dr. Diptimayee Jena Assistant Professor, Department of Biochemistry. MKCG Medical College, Berhampur, Odisha
Santosh Singh IMS & SUM Hospital, Siksha O Anusandhan (Deemed to be University)
Rajashree Panigrahy IMS & SUM Hospital, Siksha O Anusandhan (Deemed to be University)
Kundan Kumar Sahu IMS & SUM Hospital, Siksha O Anusandhan (Deemed to be University)
Dattatreya Kar IMS & SUM Hospital, Siksha O Anusandhan (Deemed to be University)
Suren Kumar Das IMS & SUM Hospital, Siksha O Anusandhan (Deemed to be University)

DOI:

https://doi.org/10.51168/sjhrafrica.v5i6.716

Keywords:

Antibiotic resistance, Antibiotic susceptibility, E.coli, ESBL, Gram-negative bacteria, K.pneumoniae

Abstract

Background

Antimicrobial-resistant organisms have led to increased mortality, morbidity, and economic burden throughout the globe. This study focused on measuring the prevalence of antimicrobial resistance bacteria mostly by extended-spectrum β-lactamase (ESBL) producers from several samples in a facility providing tertiary care in Eastern Odisha.

Methods

A cross-sectional study was conducted from February 2021 to January 2022. During that time a total of 2452 culture-positive specimens were processed from different samples. Identification of organisms and antibiotic susceptibility was done manually through Kirby Beuer’s disc diffusion method. Phenotypic detection of ESBL producers was performed by a Double disc synergy test.

Results

During the study, E. coli (852) was identified as the most prevalent organism followed by S. aureus (661) and K. pneumoniae (301). Among them, 1571 isolates were ESBL-producing and E. coli was the most prevalent one followed by S. aureus and K. pneumoniae which were 659, 479, and 172 in number respectively. Most of the ESBL producers were isolated from urine samples and the least number from stool samples. We found in this study that the highest population of P.mirabilis and K. oxytoca were resistant to the fluoroquinolones group of antibiotics, Pseudomonas and K. oxytoca are highly resistant to aminoglycosides group of antibiotics, P.mirabilis, Enterobacter, P. vulgaris and Enterococci were showing high resistance towards penicillin group of antibiotics, P.mirabilis was highly resistant towards β-lactamase inhibitor group of antibiotics.

Conclusion

As per the study findings, E. coli is the main producer of ESBLs among members of the Enterobacteriaceae family, and urine is the main source of ESBL-positive isolates. These findings are highly significant from a medical and scientific standpoint and may influence policymakers to better monitor and manage antibiotic resistance.