Isolation and identification of pathogenic bacteria present in wastewater effluents discharged into Lake Victoria at Mukuuba landing site, Wakiso district, Uganda. A cross-sectional study.
DOI:
https://doi.org/10.51168/sjhrafrica.v6i9.2040Keywords:
Isolation and Identification, Pathogenic Bacteria, Wastewater Effluents, Lake Victoria, Mukuuba Landing Site, Wakiso DistrictAbstract
Background
Wastewater effluents discharged into Lake Victoria often contain pathogenic bacteria that threaten aquatic ecosystems and public health. This study aimed to isolate and identify the pathogenic bacteria present in wastewater effluents discharged into Lake Victoria at Mukuuba Landing Site, Wakiso District, Uganda.
Methodology
The study employed a descriptive, cross-sectional, laboratory-based design and quantitative methods. A total of 30 wastewater effluent samples, purposively selected, were described using standardized microbiological techniques. Microsoft Excel 2016 was used to analyze data.
Results
Citrobacter freundii was the most frequently isolated organism (75%), followed by Enterococcus faecalis (64.29%). Staphylococcus aureus and Escherichia coli accounted for 17.86%, while Klebsiella pneumoniae (10.71%) and Proteus mirabilis (7.14%) were the least amount isolated. 70% of isolates were Gram-negative rods consistent with enterics from fecal contaminant (pink bacilli in histological image). The remaining 30% were Gram-positive cocci (purple clusters or chains), suggestive of Staphylococcus and Enterococcus species, respectively.
Conclusion
Contamination of effluent water has the potential for serious public health risks, and there is a significant risk contribution from these bacteria in wastewater effluents.
Recommendation
There should be an ideal surveillance of environmental health programs at a national level, by the Ministry of Health, to put in place the appropriate action for public health in risk zones.
References
Citrobacter. (2025). In Wikipedia. https://en.wikipedia.org/w/index.php?title=Citrobacter&oldid=1288119162
Enterococcus faecalis. (2025). In Wikipedia. https://en.wikipedia.org/w/index.php?title=Enterococcus_faecalis&oldid=1300835737
GBD 2021 Risk Factors Collaborators. (2024). Global burden and strength of evidence for 88 risk factors in 204 countries and 811 subnational locations, 1990-2021: A systematic analysis for the Global Burden of Disease Study 2021. Lancet (London, England), 403(10440), 2162–2203. https://doi.org/10.1016/S0140-6736(24)00933-4
Katumba, G., Mwanja, H., Mayito, J., Mbolanyi, B., Isaasi, F., Kibombo, D., Namumbya, J., Musoke, D., Kabazzi, J., Sekamatte, M., Idrakua, L., Walwema, R., Lamorde, M., Kakooza, F., & Etimu, S. (2024). Establishing Antimicrobial Resistance Surveillance in the Water and Environment Sector in a Resource-Limited Setting: Methodical Qualitative and Quantitative Description of Uganda’s Experience From 2021 to 2023. JMIRx Bio, 2(1), e50588. https://doi.org/10.2196/50588
Kelly, E., Cronk, R., Fisher, M., & Bartram, J. (2021). Sanitary inspection, microbial water quality analysis, and water safety in handpumps in rural sub-Saharan Africa. Npj Clean Water, 4(1), 1–7. https://doi.org/10.1038/s41545-020-00093-z
La Rosa, M. C., Maugeri, A., Favara, G., La Mastra, C., Magnano San Lio, R., Barchitta, M., & Agodi, A. (2025). The Impact of Wastewater on Antimicrobial Resistance: A Scoping Review of Transmission Pathways and Contributing Factors. Antibiotics, 14(2), Article 2. https://doi.org/10.3390/antibiotics14020131
Machado, A., Amorim, E., & Bordalo, A. A. (2022). Spatial and Seasonal Drinking Water Quality Assessment in a Sub-Saharan Country (Guinea-Bissau). Water, 14(13), Article 13. https://doi.org/10.3390/w14131987
Omohwovo, E. J. (2024). Wastewater Management in Africa: Challenges and Recommendations. Environmental Health Insights, 18, 11786302241289681. https://doi.org/10.1177/11786302241289681
Overgaard, H. J., Dada, N., Lenhart, A., Stenström, T. A. B., & Alexander, N. (2021). Integrated disease management: Arboviral infections and waterborne diarrhoea. Bulletin of the World Health Organization, 99(8), 583–592. https://doi.org/10.2471/BLT.20.269985
Schneeberger, P. H. H., Fuhrimann, S., Becker, S. L., Pothier, J. F., Duffy, B., Beuret, C., Frey, J. E., & Utzinger, J. (2019). Qualitative microbiome profiling along a wastewater system in Kampala, Uganda. Scientific Reports, 9(1), 17334. https://doi.org/10.1038/s41598-019-53569-5
Zieliński, W., Korzeniewska, E., Harnisz, M., Drzymała, J., Felis, E., & Bajkacz, S. (2021). Wastewater treatment plants as a reservoir of integrase and antibiotic resistance genes – An epidemiological threat to workers and the environment. Environment International, 156, 106641. https://doi.org/10.1016/j.envint.2021.106641
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Copyright (c) 2025 Duluga Seldon, James Kasozi , Fortunate Lujjibirwa, Daniel Manowa , Wanambwa Wamono Herbert, Akena John Baptist, Situma Christopher, Ssentongo Vianney, Muzoora Saphan, Jacqueline Kyosiimire, Florence Sulwa, Jorome Nteziyaremye , Habert Mabonga
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