MICROBIOLOGICAL SURVEILLANCE OF OPERATION THEATRES OF A TERTIARY CARE HOSPITAL IN BIHAR.

Binay Kumar Mahto KUMAR; Shreya; Sanjay Kumar; Rajesh Kumar

doi:10.51168/sjhrafrica.v5i12.1436

Authors

Binay Kumar Mahto KUMAR Bhagwan Mahavir Institute of Medical Sciences Pawapuri Nalanda
Shreya Nalanda Medical College Hospital Patna
Sanjay Kumar Nalanda Medical College Hospital Patna
Rajesh Kumar Nalanda Medical College Hospital Patna

DOI:

https://doi.org/10.51168/sjhrafrica.v5i12.1436

Keywords:

Microbial surveillance, operation theaters, settle plate method

Abstract

Background

Microbial contamination of air and environment in operation theatres (OTs) has continued to increase the prevalence of surgical site and hospital-associated infections. Control of these infections in operation theatres is of utmost importance as it can increase patient’s morbidity and mortality.

Aims and Objectives

Aim of the present study was to identify bacterial colonization of surfaces and to determine the microbial contamination of air by estimating the bacterial colony forming unit (CFU) rate in the OT’s of a tertiary care hospital.

Material and Method

This was the tertiary care hospital based retrospective observational study done for a period of 18 months. Data was collected from routine microbiological surveillance of OT’s done between February 2023 to July 2024. Two sampling procedures were considered in this study. For air quality surveillance, settle plate method whereas for surface sampling, soaked swabs from different sites in peptone water were included. Collected samples were transported to Microbiology Laboratory of the institution in sterile condition and careful observation was done of bacterial species, which were isolated and further identified by conventional methods.

Results

A total of 463 samples were collected from different surfaces of various OT’s. Out of these 130 samples showed bacterial growth. The predominant species isolated was Aerobic Spore-Forming Bacilli with 87/130 isolates followed by coagulase-negative Staphylococcus 31/130 , Klebsiella species 8/130 , E.coli 2/130 , Staphylococcus aureus 1/130 & Pseudomonas aeruginosa 1/130.

OT’s air samples showed highest colony forming unit (cfu) rate in Labor room (133cfu/m3) and least cfu rate in Radiotherapy (38cfu/m3).

Conclusions

OTs of our hospital showed highest bacterial contamination in Labor room where the patient load was high and among all the surfaces, OT tables and floor were mostly contaminated with microorganisms.

Author Biographies

Shreya , Nalanda Medical College Hospital Patna

Post graduate trainee, Department of Microbiology, Nalanda Medical College Hospital Patna

Sanjay Kumar , Nalanda Medical College Hospital Patna

Professor and Head, Department of Microbiology, Nalanda Medical College Hospital Patna

Rajesh Kumar , Nalanda Medical College Hospital Patna

Professor, Department of Microbiology, Nalanda Medical College Hospital Patna

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