Immunohistochemical expression of CD44 in Colorectal carcinoma in relation to histomorphologic parameters and clinico-pathological factors

Authors

  • Dr. Pragnya Paramita Mishra Associate Professor, Department of pathology, Hitech medical college, Rourkela, India
  • Dr. Madan K Assistant Professor, Department of Oncopathology, Yenepoya Medical College, Mangalore, India
  • Dr. Anuradha Calicut Kini Rao Professor, Department of Pathology, Kasturba Medical college, Mangalore, India
  • Dr. Siddhartha Biswas Professor & HOD, Department of Oncopathology, Yenepoya Medical College, Mangalore, India
  • Dr. Rohan Shetty Professor, Department of Oncology,Yenepoya Medical College, Mangalore, India
  • Premanand Panda HOD, Depatment of Radiology, JP hospital, Rourkela, India

DOI:

https://doi.org/10.51168/sjhrafrica.v5i6.1240

Keywords:

Colorectal carcinoma, CD44, Immuhistochemistry, Stromal cell, Stem Cell Marker

Abstract

Background: Cancer stem cells (CSC) have proven to play a vital role in cell invasion, metastasis, and treatment resistance in colorectal carcinoma (CRC), which subsequently led to poor outcomes. Cluster of differentiation 44 (CD44) is usually expressed in stem cells in CRC’s and can be detected by immunohistochemistry (IHC).

Aims: The aim of this study is to evaluate the immunohistochemical expression of cancer stem cell markers CD44 in colorectal cancer and investigate their correlation with different clinicopathological characters of prognostic significance.

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Methods: A cross-sectional study included 52 patients with primary CRC were analyzed for CD44 expression by IHC on paraffin embedded blocks. Data were collected, tabulated, and statistically analyzed by using SPSS Version 23.0.

Results: This survey found a male predominance over 45. 41 (78.8%) of CRC cases had membranous CD44 positivity in cancer cells and 31 (59.6%) in stromal cells. Signet ring cells have modest membranous CD 44 positivity. CD44 expression was unrelated to age, sex, or laterality. Regardless of physical appearance, tumour cells expressed CD44, especially annular tumours. Tumour budding was associated with higher tumour stages (T3, T4), greatest in T4, and tumour sizes over 5cm. It did not correspond with nodal stage or perineural and lymphovascular invasion. CD44 expression did not affect tumour budding grade. Regardless of morphological criteria (gross or microscopic), stromal CD44 expression was not correlated.

Conclusions: CRC prognosis is independently correlated with CD44 expression, a stem cell marker. They are linked to epithelial-mesenchymal transition (EMT) and tumour budding, with increased expression in high-burden instances.

Recommendation: Further research should be conducted on the role of CD44 expression in colorectal cancer, particularly focusing on post-neoadjuvant chemotherapy cases, to better understand its prognostic implications and potential as a therapeutic target.

Published

2024-06-30

How to Cite

Mishra, D. P. P. ., K, D. M. ., Rao, D. A. C. K. ., Biswas, D. S. . . ., Shetty, D. R. ., & Panda, P. . (2024). Immunohistochemical expression of CD44 in Colorectal carcinoma in relation to histomorphologic parameters and clinico-pathological factors. Student’s Journal of Health Research Africa, 5(6). https://doi.org/10.51168/sjhrafrica.v5i6.1240

Issue

Section

Section of Radiology and Radiotherapy